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Santa Cruz Biotechnology
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Santa Cruz Biotechnology
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Becton Dickinson
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Image Search Results
Journal: Oncogene
Article Title: Tissue-specific alternative splicing in the human INK4a/ARF cell cycle regulatory locus.
doi: 10.1038/sj.onc.1202737
Figure Lengend Snippet: Figure 5 (a) Immunoprecipitation (IP)-Western blotting of transfected cells. PANC-1 cells (INK4a7/ARF7) and C-33A cells (INK4a+/ARF+) were transfected with expression vectors for p12 (p12senseNeo), INK4a (p16senseNeo, PANC-1 only), or empty vector (pcDNA3.1) followed by immunoprecipitation with an INK4a exon 1a-speci®c antibody and Western detection with the same antibody to con®rm that the respective proteins were made in vivo. (b) Immunoprecipitations in C-33A cells transfected with the indicated expression vectors or mock transfected. The pulldown antibody (to cdk4 or an irrelevant protein (Ir), the Sp1 transcription factor) and the subsequent Western detection antibody are indicated above. The left-most portion shows the same controls from part A while the right-most panel is a positive control for the detection of cdk4 in C-33A cells. Molecular weights, in kiloDaltons (kD), are indicated and the position of each protein is indicated at the right
Article Snippet: Rabbit anti-INK4a 18 antibody (N-20, Santa Cruz, USA) was used at a dilution of 1 : 100 and
Techniques: Immunoprecipitation, Western Blot, Transfection, Expressing, Plasmid Preparation, In Vivo, Positive Control
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: Screening of anti-phosphoT172 CDK4 mAbs using immobilized cyclin D3/CDK4 proteic fusions.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques:
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: Sera evaluation by CDK4 peptide ELISA.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques: Peptide ELISA
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: Evaluation of CDK4 T172-phosphospecificity of immune sera.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques:
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: CDK4 T172-phosphospecificity of cloned mAbs.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques: Clone Assay
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: T172-phosphospecific CDK4 sandwich ELISA.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques: Sandwich ELISA
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: S130-phosphorylated p21 is enriched in phospho-CDK4 immunoprecipitation.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques: Immunoprecipitation
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: S10-phosphorylated p27 is enriched in phospho-CDK4 immunoprecipitation.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques: Immunoprecipitation
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: S10-phosphorylated p27 is enriched in phospho-CDK4 immunoprecipitation.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27, CDK4 antibodies (respectively 12D1, D69C12, D9G3E, Cell Signaling Technology); or rabbit polyclonal (H22) CDK4 and
Techniques: Immunoprecipitation
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: S130-phosphorylated p21 is enriched in phospho-CDK4 immunoprecipitation.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (
Techniques: Immunoprecipitation
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: Screening of anti-phosphoT172 CDK4 mAbs using immobilized cyclin D3/CDK4 proteic fusions.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher);
Techniques:
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: S10-phosphorylated p27 is enriched in phospho-CDK4 immunoprecipitation.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21,
Techniques: Immunoprecipitation