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Image Search Results
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: Screening of anti-phosphoT172 CDK4 mAbs using immobilized cyclin D3/CDK4 proteic fusions.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27, CDK4 antibodies (respectively 12D1, D69C12, D9G3E,
Techniques:
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: Sera evaluation by CDK4 peptide ELISA.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27, CDK4 antibodies (respectively 12D1, D69C12, D9G3E,
Techniques: Peptide ELISA
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: Evaluation of CDK4 T172-phosphospecificity of immune sera.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27, CDK4 antibodies (respectively 12D1, D69C12, D9G3E,
Techniques:
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: CDK4 T172-phosphospecificity of cloned mAbs.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27, CDK4 antibodies (respectively 12D1, D69C12, D9G3E,
Techniques: Clone Assay
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: T172-phosphospecific CDK4 sandwich ELISA.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27, CDK4 antibodies (respectively 12D1, D69C12, D9G3E,
Techniques: Sandwich ELISA
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: S130-phosphorylated p21 is enriched in phospho-CDK4 immunoprecipitation.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27, CDK4 antibodies (respectively 12D1, D69C12, D9G3E,
Techniques: Immunoprecipitation
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: S10-phosphorylated p27 is enriched in phospho-CDK4 immunoprecipitation.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27, CDK4 antibodies (respectively 12D1, D69C12, D9G3E,
Techniques: Immunoprecipitation
Journal: Molecular and Cellular Biology
Article Title: c-Myc or Cyclin D1 Mimics Estrogen Effects on Cyclin E-Cdk2 Activation and Cell Cycle Reentry
doi: 10.1128/mcb.18.8.4499
Figure Lengend Snippet: FIG. 3. Cdk4 activity following E2 treatment or Zn induction of c-Myc or cyclin D1. The experimental design was as described for Fig. 2A. (A) Cdk4 immunoprecipitates were assayed for kinase activity toward a GST-pRB773-928
Article Snippet: Cdk4 complexes were immunoprecipitated by incubating lysates containing 400 mg of protein with 5 ml of a
Techniques: Activity Assay
Journal: Cancer research
Article Title: Mitogenic Action of the Androgen Receptor Sensitizes Prostate Cancer Cells to Taxane-Based Cytotoxic Insult
doi: 10.1158/0008-5472.CAN-06-2249
Figure Lengend Snippet: The synergistic effect of paclitaxel and AR activation is attributed to enhanced apoptosis. A, LNCaP cells were seeded on coverslips in CDT + EtOH, FBS, or CDT + 10−10 mol/L DHT. The cells were exposed to 10−6 mol/L paclitaxel (PTX) or DMSO control for 24 hours. Cells were fixed and nuclei were stained with Hoechst DNA dye. Left, representative images; right, percentage micronucleated nuclei from at least three experiments done in triplicate. B, LNCaP cells were seeded in CDT + EtOH, FBS, or CDT + 10−10 mol/L DHT, and adherent and floating cells were collected after 24 hours of 10−6 mol/L paclitaxel or DMSO control. Isolated protein was immunoblotted with an antibody specific for both full-length and cleaved PARP (106 and 89 kDa, respectively). CDK4 is the loading control. C, LNCaP cells were seeded as described. One hour before paclitaxel exposure, 50 µmol/L Ac-VAD-CHO pan-caspase inhibitor was added to the medium. Following 24 hours of 10−6 mol/L paclitaxel treatment in the presence of Ac-VAD-CHO, cell survival was determined as described previously. D, LNCaP cells were seeded, harvested, and collected as in (B). Isolated protein was immunoblotted with the following: (left) antisera against p53, Ser15 p53, bcl-2, and Bax (numbers correspond to fluorescence intensity of Ser15 p53 relative to no paclitaxel control in the same condition); (middle) antisera against p21CIP1 and Hsp27; (right) antisera against E2F-1.
Article Snippet: The following antibodies were used: rabbit polyclonal poly(ADP-ribose) polymerase (PARP) and Ser 15 p53 phosphorylated specific (Cell Signaling, Danvers, MA), Bax (N-20) and cyclin-dependent kinase (CDK) 4 (H-22; Santa Cruz Biotechnology, Santa Cruz, CA), E2F-1 (Ab-3; NeoMarkers), p53 (Ab-6; Calbiochem), and
Techniques: Activation Assay, Staining, Isolation, Fluorescence
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: Screening of anti-phosphoT172 CDK4 mAbs using immobilized cyclin D3/CDK4 proteic fusions.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques:
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: Sera evaluation by CDK4 peptide ELISA.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques: Peptide ELISA
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: Evaluation of CDK4 T172-phosphospecificity of immune sera.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques:
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: CDK4 T172-phosphospecificity of cloned mAbs.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques: Clone Assay
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: T172-phosphospecific CDK4 sandwich ELISA.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques: Sandwich ELISA
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: S130-phosphorylated p21 is enriched in phospho-CDK4 immunoprecipitation.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques: Immunoprecipitation
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: S10-phosphorylated p27 is enriched in phospho-CDK4 immunoprecipitation.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27,
Techniques: Immunoprecipitation
Journal: Cell Cycle
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
doi: 10.1080/15384101.2021.1984663
Figure Lengend Snippet: S130-phosphorylated p21 is enriched in phospho-CDK4 immunoprecipitation.
Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology);
Techniques: Immunoprecipitation